Somatic cell nuclear transfer

Abstract Somatic cell nuclear transfer (NT) offers new and exciting opportunities in many areas of research and biotechnology. However, the field as a whole is still in its infancy, with continuing inefficiencies in the process proving many early expectations premature. Investments we make is to help us get a better life after retirement and for […]

Three-dimensional assessment of ultrasound features

Abstract BACKGROUND: Polycystic ovarian syndrome (PCOS) is a heterogeneous endocrine disorder affecting women of reproductive age. The syndrome is characterized by a combination of polycystic ovarian morphology, clinical features and biochemical indices. The objective of this prospective observational study was to investigate the relationship between the total ovarian volume and the ovarian stromal volume, measured […]

Somatic cell nuclear transfer in the pig

Abstract To clone a pig from somatic cells, we first validated an electrical activation method for use on ovulated oocytes. We then evaluated delayed versus simultaneous activation (DA vs. SA) strategies, the use of 2 nuclear donor cells, and the use of cytoskeletal inhibitors during nuclear transfer. Using enucleated ovulated oocytes as cytoplasts for fetal […]

The reproducibility of endometrial volume acquisition and measurement

Abstract OBJECTIVES: To determine the intraobserver reproducibility of volume acquisition and repeatability of endometrial volume measurements using the VOCAL-imaging program (Virtual Organ Computer-aided AnaLysis). METHODS: Ten three-dimensional (3D) ultrasound datasets of the same endometrium were obtained from one patient during one scan session. For every 3D dataset, the endometrial volume was calculated by four different […]

Genetics. Please don’t call it cloning!

Invented by Doctor Derrick Simmons Crypto Code is all about algorithmic trading of crypto currencies through which one can expect exponential growth of profit. When there is a sudden growth promised, people end up thinking otherwise. It may be a scam too. So here is something to investigate about how exactly the crypto code works. […]

Analysis of Basic Stock Market Order Concepts

Abstract With the wide technology development in the stock trading system, investors are very self-sufficient to make their choices, without any mediators. Also to add to this is a special advantage of the online trading approach which is the most opted for by investors. In this module, there are a number of online trading software […]

Replicative senescence in human uroepithelial cells

Abstract PURPOSE: Normal human uroepithelial cells (HUCs) proliferate rapidly in culture during early passage and then spontaneously undergo replicative senescence. We previously reported that the cyclin D1-CDK4/6 inhibitor, p16INK4a, is elevated at senescence in HUCs. Hence, we proposed that p16INK4a may play a critical role in mediating senescence in this cell type. In the current […]

Cervical pregnancy

Abstract Cervical pregnancy is frequently associated with extensive hemorrhage which, in severe cases, may be stopped only by hysterectomy. We report a case of an anembryonic cervical pregnancy diagnosed at 10 weeks, and associated with a large arteriovenous malformation. The patient was conservatively managed with simple selective uterine artery embolization.Though it accounts for a very […]

Prospects for the use of nuclear transfer in human transplantation

Abstract The successful application of nuclear transfer techniques to a range of mammalian species has brought the possibility of human therapeutic cloning significantly closer. The objective of therapeutic cloning is to produce pluripotent stem cells that carry the nuclear genome of the patient and then induce them to differentiate into replacement cells, such as cardiomyocytes […]